Reminder: Dr. Rulin on conference call tonight. (Tuesday) 6 p.m. PST 7 p.m. MST 8 p.m. CST 9 p.m. EST
Pin Code: 93275#
Question from prospective customer/member: Another thing we need to find out . . . is the Calcium . . . too much without Magnesium is not good for the health . . . I did not see any Magnesium in this pearl product.
Reply by Dr. Rulin: Pearlcium contain magnesium and more than 26 essential trace minerals.
Pearl is mainly composed of calcium carbonate, a product of calcium metabolism among bi-valves. The formation of pearl is thought to be dependent on a protein process that involves signal proteins. Signal proteins in pearl are a group of proteins that participate in, control and regulate calcium uptake, transportation and secretion in the process of pearl formation 1-37
In a study published in July 2004, Dr. S. Li and his colleagues isolated part of the DNA of pearl that can reproduce a complete full-length of one of the signal proteins.1 To their amazement, they found that the pearl signal protein DNA encodes a protein which shares high similarity with our own human calcium binding proteins.
The research done by Dr. Li and his colleagues, as well as many other scientists, indicates that the regulation of calcium uptake, transport, and secretion involved in creating pearl is similar to the regulation of calcium uptake, transport, and absorption in the human body — right on down to the DNA level.1-37 Wow! Consider the notion that bone formation is just a human adaptation of pearl formation — one that is happening right inside you. It’s more truth than fantasy, so I hope you feel even more precious now.
Who would have guessed that the formation, regulation and overall calcium metabolism of the human skeleton is so similar to that of the precious pearl’s? We share a deep-rooted kinship, a connection at the DNA level, a true evolutionary bond with this luminous treasure. Of course, this may also account for why pearl is so compatible and osteogenic for our human bones and at the same time, nutritious and healing for so much of the human body. It is really little wonder that we have been so drawn to pearl throughout the ages.
1. Li S, Xie L, Zhang C, Zhang Y, Gu M, Zhang R.: “Cloning and expression of a pivotal calcium metabolism regulator: calmodulin involved in shell formation from pearl oyster (Pinctada fucata).” Comp Biochem Physiol B Biochem Mol Biol. 2004 Jul; 138(3):235-43.
2. Duplat D, Puisségur M, Bédouet L, Rousseau M, Boulzaguet H, Milet C, Sellos D, Van Wormhoudt A, Lopez E. “Identification of calconectin, a calcium-binding protein specifically expressed by the mantle of Pinctada margaritifera.”, FEBS Lett. 2006 May 1;580(10):2435-41. Epub 2006 Apr 7.
3. Shen X, Belcher AM, Hansma PK , Stucky GD, Morse DE.: “Molecular cloning and characterization of lustrin A, a matrix protein from shell and pearl pearl of Haliotis rufescens.” J Biol Chem. 1997 Dec 19;272(51): 32472-81.
4. Zhang Y, Xie L, Meng Q, Jiang T, Up R, Chen L, Zhang R. “A novel matrix protein participating in the pearl framework formation of pearl oyster, Pinctada fucata.” Comp Biochem Physiol B Biochem Mol Biol. 2003 Jul; 135(3): 565-73.
5. Tsukamoto D, Sarashina I, Endo K: “Structure and expression of an unusually acidic matrix protein of pearl oyster shells.” Biochem Biophys Res Commun. 2004 Aug 6;320(4):1175-80.
6. Michenfelder M, Fu G, Lawrence C, Weaver JC, Wustman BA, Taranto L, Evans JS, Morse DE.: “Characterization of two molluscan crystal-modulating biomineralization proteins and identification of putative mineral binding domains.” Biopolymers. 2003 Dec; 70(4):522-33.
7. Blank S, Arnoldi M, Khoshnavaz S, Treccani L, Kuntz M, Mann K, Grathwohl G, Fritz M., “The pearl protein perlucin nucleates growth of calcium carbonate crystals.” J Microsc. 2003 Dec; 212(Pt 3):280-91
8. Miyamoto H, Miyoshi F, Kohno J.: “The carbonic anhydrase domain protein pearlin is expressed in the epithelial cells of the mantle and acts as a negative regulator in calcification in the mollusc Pinctada fucata.” Zoolog Sci. 2005 Mar;22(3):311-5.
9. Yan Z, Fang Z, Ma Z, Deng J, Li S, Xie L, Zhang R, “Biomineralization: functions of calmodulin-like protein in the shell formation of pearl oyster.” Biochim Biophys Acta. 2007 Sep;1770(9):1338-44. Epub 2007 Jul
10. Takakura D, Norizuki M, Ishikawa F, Samata T., “Isolation and Characterization of the N-linked Oligosaccharides in Pearlin from Pinctada fucata.” Mar Biotechnol (NY). 2008 Feb 6
11. Wang N, Lee YH, Lee J., “Recombinant perlucin nucleates the growth of calcium carbonate crystals: Molecular cloning and characterization of perlucin from disk abalone, Haliotis discus discus.” Comp Biochem Physiol B Biochem Mol Biol. 2008 Feb;149(2):354-61. Epub 2007 Oct
12. Yan Z, Jing G, Gong N, Li C, Zhou Y, Xie L, Zhang R. “N40, a novel nonacidic matrix protein from pearl oyster pearl, facilitates nucleation of aragonite in vitro.” Biomacromolecules, 2007 Nov;8(11):3597-601. Epub 2007 Oct
13. Fan W, Li C, Li S, Feng Q, Xie L, Zhang R, “Cloning, characterization, and expression patterns of three sarco/endoplasmic reticulum Ca2+-ATPase isoforms from pearl oyster (Pinctada fucata)”, Acta Biochim Biophys Sin (Shanghai). 2007 Sep;39(9):722-30
14. Yano M, Nagai K, Morimoto K, Miyamoto H, “A novel pearl protein N19 in the pearl oyster Pinctada fucata.”, Biochem Biophys Res Commun. 2007 Oct 12;362(1):158-63. Epub 2007 Aug
15. Fan W, Li C, Wang X, Gong N, Xie L, Zhang R., “Cloning, characterization and expression analysis of calcium channel beta subunit from pearl oyster (Pinctada fucata).”, J Biosci Bioeng. 2007 Jul;104(1):47-54.
16. Metzler RA, Abrecht M, Olabisi RM, Ariosa D, John son CJ, Frazer BH, Coppersmith SN, Gilbert PU., “Architecture of columnar pearl, and implications for its formation mechanism.” Phys Rev Lett. 2007 Jun 29;98(26):268102. Epub 2007 Jun 29.
17. Xie LP, Wu YT, Dai YP, Li Q, Zhang RQ. “A novel glycosylphosphatidylinositol-anchored alkaline phosphatase dwells in the hepatic duct of the pearl oyster, Pinctada fucata.” Mar Biotechnol (NY). 2007 Sep-Oct;9(5):613-23. Epub 2007 Jul 13
18. Suzuki M, Sakuda S, Nagasawa H, “Identification of chitin in the prismatic layer of the shell and a chitin synthase gene from the Japanese pearl oyster, Pinctada fucata.” Biosci Biotechnol Biochem. 2007 Jul;71(7):1735-44. Epub 2007 Jul
19. Lin AY, Chen PY, Meyers MA, “The growth of pearl in the abalone shell.” Acta Biomater. 2008 Jan;4(1):131-8. Epub 2007 Jul 9
20. Ma Z, Huang J, Sun J, Wang G, Li C, Xie L, Zhang R, “A novel extrapallial fluid protein controls the morphology of pearl lamellae in the pearl oyster, Pinctada fucata.” J Biol Chem. 2007 Aug 10;282(32):23253-63. Epub 2007 Jun 8
21. Huang J, Zhang C, Ma Z, Xie L, Zhang R, “A novel extracellular EF-hand protein involved in the shell formation of pearl oyster.” Biochim Biophys Acta. 2007 Jul;1770(7):1037-44. Epub 2007 Mar 20
22. Liu HL, Liu SF, Ge YJ, Liu J, Wang XY, Xie LP, Zhang RQ, Wang Z, “Identification and characterization of a biomineralization related gene PFMG1 highly expressed in the mantle of Pinctada fucata.”, Biochemistry. 2007 Jan 23;46(3):844-51.
23. Naganuma T, Ogawa T, Hirabayashi J, Kasai K, Kamiya H, Muramoto K, “Isolation, characterization and molecular evolution of a novel pearl shell lectin from a marine bivalve, Pteria penguin.” Mol Divers. 2006 Nov;10(4):607-18. Epub 2006 Nov 17
24. Zhang C, Li S, Ma Z, Xie L, Zhang R., “A novel matrix protein p10 from the pearl of pearl oyster (Pinctada fucata) and its effects on both CaCO3 crystal formation and mineralogenic cells.”, Mar Biotechnol (NY). 2006 Nov-Dec;8(6):624-33. Epub 2006 Sep 18
25. Asakura T, Hamada M, Ha SW, Knight DP., “Conformational study of silklike peptides modified by the addition of the calcium-binding sequence from the shell pearlous matrix protein MSI60 using 13C CP/MAS NMR spectroscopy.” Biomacromolecules. 2006 Jun;7(6):1996-2002
26. Duplat D, Puisségur M, Bédouet L, Rousseau M, Boulzaguet H, Milet C, Sellos D, Van Wormhoudt A, Lopez E., “Identification of calconectin, a calcium-binding protein specifically expressed by the mantle of Pinctada margaritifera.”, FEBS Lett. 2006 May 1;580(10):2435-41. Epub 2006 Apr 7
27. Li S, Xie L, Ma Z, Zhang R, “cDNA cloning and characterization of a novel calmodulin-like protein from pearl oyster Pinctada fucata.” FEBS J. 2005 Oct;272(19):4899-910
28. Wang XX, Xie L, Wang R., “Biological fabrication of pearlous coating on titanium dental implant”, Biomaterials. 2005 Nov;26(31):6229-32.
29. Miyamoto H, Miyoshi F, Kohno J., “The carbonic anhydrase domain protein pearlin is expressed in the epithelial cells of the mantle and acts as a negative regulator in calcification in the mollusc Pinctada fucata.”, Zoolog Sci. 2005 Mar;22(3):311-5.
30. Li S, Xie L, Zhang C, Zhang Y, Gu M, Zhang R., “Cloning and expression of a pivotal calcium metabolism regulator: calmodulin involved in shell formation from pearl oyster (Pinctada fucata).”, Comp Biochem Physiol B Biochem Mol Biol. 2004 Jul;138(3):235-43
31. Tsukamoto D, Sarashina I, Endo K., “Structure and expression of an unusually acidic matrix protein of pearl oyster shells,” Biochem Biophys Res Commun. 2004 Aug 6;320(4):1175-80.
32. Suzuki M, Murayama E, Inoue H, Ozaki N, Tohse H, Kogure T, Nagasawa H, “Characterization of Prismalin-14, a novel matrix protein from the prismatic layer of the Japanese pearl oyster (Pinctada fucata)”, Biochem J. 2004 Aug 15;382(Pt 1):205-13
33. Matsushiro A, Miyashita T, Miyamoto H, Morimoto K, Tonomura B, Tanaka A, Sato K., “Presence of protein complex is prerequisite for aragonite crystallization in the pearlous layer.” Mar Biotechnol (NY). 2003 Jan-Feb;5(1):37-44
34. hang Y, Xie L, Meng Q, Jiang T, Pu R, Chen L, Zhang R., “A novel matrix protein participating in the pearl framework formation of pearl oyster, Pinctada fucata.”, Comp Biochem Physiol B Biochem Mol Biol. 2003 Jul;135(3):565-73.
35. Samata T, Hayashi N, Kono M, Hasegawa K, Horita C, Akera S, “A new matrix protein family related to the pearlous layer formation of Pinctada fucata.”, FEBS Lett. 1999 Nov 26;462(1-2):225-9.
36. Shen X, Belcher AM, Hansma PK, Stucky GD, Morse DE, “Molecular cloning and characterization of lustrin A, a matrix protein from shell and pearl pearl of Haliotis rufescens.”, J Biol Chem. 1997 Dec 19;272(51):32472-81.
37. Bédouet L, Marie A, Dubost L, Péduzzi J, Duplat D, Berland S, Puisségur M, Boulzaguet H, Rousseau M, Milet C, Lopez E., “Proteomics analysis of the pearl soluble and insoluble proteins from the oyster Pinctada margaritifera”, Mar Biotechnol (NY). 2007 Sep-Oct;9(5):638-49. Epub 2007 Jul 21.
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